In the study DADS was selected as material， and the antibacterial activities and the minimal inhibitory concentration of DADS against non-O157 Shiga toxin-producing Escherichia coli CICC10670 were determined. Real-time quantitative reverse transcriptase-polymerase chain reaction was performed to evaluate the effect of DADS on the virulence factors stx2， eaeA and ehxA of E. coli. The effect of DADS as an inhibitor on the motility of Escherichia coli. The result showed that DADS can the higher inhibitory effect against E. coli. Subinhibitory concentrations of DADS inhibited the expression of stx2， eaeA and ehxA. The relative expression decreased to more than 85%， and a dose-dependent attenuation of stx2， eaeA and ehxA production was observed. DADS inhibited the ability of E. coli to swim in a dose-dependent manner at sub MIC concentrations. In summary， DADS has good antibacterial effect on the pathogenic E. coli and low concentration will inhibit expression of the virulence genes， DADS is suitable as a potential natural preservative， preventing food poisoning and extending the shelf life of food.