This paper reported the effects of compound enzyme on Acaudina molpadioides with the aim of tenderization. The results showed animal protein hydrolytic enzyme had the best effects in hydrolysis and the neutral protease had a light smell. The optimum parameters were as follows： amount of Acaudina molpadioides 4 g/100 mL， 40 ℃， ration of enzyme 1 ∶ 1， dosage of enzyme 6×103 IU/100 mL， pH 7， 1 h. After been proposed by compound enzyme， there were great changes in structure in protein fibers in Acaudina molpadioides， the protein fibers became loose， and the density of external layer structure reduced. On the contrary， though the content of polysaccharide decreased lightly， the content of saponin in Acaudina molpadioides， which was regarded as a biological material， doubled in growth after been proposed by compound enzyme.