As a common human pathogen， Staphylococcus aureus （S. aureus） can contaminate food through various means and lead to S. aureus food poisoning （SFP）. The secretion of Staphylococcal enterotoxins （SE） in S. aureus can greatly increase its invasion and pathogenicity. By comparing the carrying and expression of the SE genes， it’s aimed to lay a foundation for further analysis of the relationship between the expression of SE genes and environmental conditions， so as to provide theoretical and data support for SFP prevention and control. In this study， PCR and 3MTMTecraTM Staph enterotoxin ID test were used to detect the carrying and expression of 5 traditional SE （SEA-SEE） genes in 33 foodborne S. aureus isolates. The results showed that the detection rate of SEs genes was 100%， with the highest and lowest carrying rates of seb （48.48%， 16/33） and see （9.09%， 3/33）， respectively. Only 63.64% （21/33） of these isolates had expressed SEs. It is proved that the carrying of SEs doesn’t mean its correct expression of the toxin protein； corresponding system regulation and appropriate environmental factors may be also needed.