Lipid， which has many biological functions and nutritional functions， is one of the major food nutrients. There are many conventional methods in the analysis of lipid separation and analysis. As an emerging method， the silver ion-composite complexation technology has better selectivity， and can improve the yield and purity of the product. This paper mainly reviews the research status of the silver ion-composite complexation technology in the separation and analysis of lipid from three aspects. They are the mechanism of the silver ion-composite complexation technology， the methods of extracting lipids with silver ions， and the application of silver ions in different biological samples， respectively. In the meantime， it also explores the huge development space of silver ion-composite complexation technology in the separation and analysis of lipids.