The aim of this study was to investigate the anti-oxidative effects of WNWAD， WNW， WAD， WN and AD against the oxidative stress induced by H2O2 in HEK-293 cells， including ORAC， GSH and GSSG levels and the effects on gene expressions and activity of γ-GCS. Oxygen radical absorbance capacity（ORAC） results showed that 5.0 μmol/L WNWAD， WAD， WNW and WN had different oxygen radical absorption capacities in vitro but AD. Then， on the base of oxidative stress model of HEK-293cells， GSH and GSSG assay results indicated that HEK-293 cells induced by H2O2 decreased GSH level from （173.52 ± 2.87） μmol/L to （115.85 ± 2.03） μmol/L （P<0.01）， and increased GSSG level from （0.90 ± 0.08） μmol/L to （33.23 ± 0.68） μmol/L （P<0.01）. Egg white peptides （except AD） can obviously increase GSH level （P<0.05） and decrease GSSG lever（P<0.01）， especially 0.5 μmol/L WNWAD decreased GSSG lever in HEK-293 cells from （33.23 ± 0.68） μmol/L to （0.96 ± 0.02） μmol/L （P<0.01） and increased GSH/GSSG ratio from 3.49 ± 0.68 to 174.22 ± 0.13 （P<0.01）. In addition， peptides on the expression of γ-GCS mRNA result showed that the peptides improved the expression of γ-GCS. Among them， the highest expression of γ-GCS mRNA in the 5.0 μmol/L WN-treated cells was 8.53 ± 0.06. All the above results suggested that egg white peptides protected HEK-293 cells against H2O2-induced oxidative stress by absorbing ROS to decrease the consumption of GSH and inhibiting GSH/ GSSG ratio changed dramatically. Egg white peptides also can upregulate gene expressions of γ-GCS， which could make the synthesis of GSH increase to against H2O2-induced oxidative stress.