Volatile compounds of navel orange in Gannan and other areas by gas chromatography-mass spectrometry （GC-MS）， and the metabolomics of navel orange in different areas was studied， which provided scientific basis for quality assurance of Gannan navel orange. The volatile components of navel orange were analyzed by GC-MS and identified by database matching with the relevant reports. The potential biomarkers were screened by the s-plot of orthogonal partial least squares discriminant analysis （OPLS-DA） and the variable importance in the projection value （VIP>1， P<0.05）. The results showed that 66 volatile components were identified in Gannan navel orange， which were terpenes， aldehydes， alcohols， esters and ketones， the highest content was D-limonene. The five volatile components were （1S）-（+）-3-Carene，4-Carene， nonanal， （Z）-Caryophyllene， Eudesma-3，7（11）-diene， which can serve as potential biomarkers for the distinction between navel orange and other regions of the navel orange.