Pulsed light is a novel non-thermal sterilization technology. In order to study and predict the sterilization effect of pulsed light on Aspergillus flavus （A. flavus） spores， A. flavus spores on solid medium were treated by pulsed light with intensities of 2.86， 1.60， 1.08 and 0.93 W/cm2 from 1 s to 60 s. Micro morphology of the spores with and with out treatments were observed by scanning electron microscope（SEM）. Log-linear model， Weibull model and Weibull+tail model were used to fit the kinetics model. The results showed that with the increase of irradiation intensity and time， the inactivation effect of pulsed light on A. flavus spores was enhanced. After 19 s of treatment under intensity of 2.86 W/cm2， all the spores with initial population of 5.15 lg（CFU/mL） were inactivated. SEM demonstrated the structure of spores collapsed after the treatment. Compared with the Log-linear model， both Weibull+tail model and Weibull model could fit the inactivation curves better （R2>0.95）. But Weibull+tail model was more accurate （R2=0.9727）.