The chlorogenic acid reference material was prepared using honeysuckle as raw material by the method of water extraction and ethanol precipitation combined with medium pressure preparative liquid chromatography and further analyzed and identified. Firstly， the identification on chemical structure of the obtained reference material was carried out by FT-IR spectroscopy， UV spectroscopy， MS， NMR H and C spectra. Furthermore， the homogeneity （inter- and intra-bottle difference） and short-term （one week， room temperature， 4 ℃， -20 ℃） and long-term （six months， -20 ℃） stability of the obtained standard reference material were evaluated using HPLC with UV detector. Finally， the multi-lab joint determination method was used to certificate the reference substance and the uncertainty of the certification results was evaluated. The obtained experimental results show that the purity value of the prepared chlorogenic acid is above 99%， and the effective period is at least 6 months （-20 ℃）. This reference chlorogenic acid has high purity， good homogeneity and stability， and can be used for confirming and evaluating the analysis method of chlorogenic acid in the honeysuckle.