In order to evaluate the effect of heavy metal artificial antigen， heterocyclic bifunctional chelators（BFCs），2-S-（4-Aminobenzyl）-1，4，7-triazacyclononane-1，4，7-triacetic acid（P-NH2-Bn-NOTA）and heavy metal Pb2+ were selected. The Pb2+ was conjugated to bovine serum albumin （BSA） and ovalbumin （OVA） with glutaraldehyde method to produce the artificial antigen based on the bifunctional chelators NOTA， respectively. The conjugation ratio was 9 and 7 for the Pb2+-NOTA-BSA conjugate and the Pb2+-NOTA-OVA conjugate， respectively. The polyclonal antibody （PcAb） was obtained after immune rabbits. Based on the PcAb， an indirect ELISA was established and its linear regression equation was y=12.127ln（x）-11.386 （R2=0.9961）. According to the ELISA， the value of detection limit （IC20） and IC50 were 13.30 and 157.89 ng/mL， respectively. The specificity analysis showed that the cross-reactivities（CR） were all less 5.3% except Cd2+ was 12.3%， which indicated that the PcAb was specific for Pb2+. The produced Pb2+ artificial antigen and the PcAb could lay the foundation for rapid analysis of heavy metal residue using the BFCs.