母乳婴儿源乳杆菌的筛选及其免疫调节能力研究
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河北省自然科学基金项目(C2017204094)


Screening of Potential Probiotic Lactobacillus and Its Research on Immunomodulatory Activity in Vitro from Breast-fed Infant Faeces
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    摘要:

    以分离自母乳婴儿源的乳杆菌——鼠李糖乳杆菌BF-1、植物乳杆菌BF-15、唾液乳杆菌BF-29、干酪乳杆菌BF-55为对象,研究这些菌株对体外模拟人工胃、肠液及胆盐的耐受性,对Caco-2细胞的黏附能力、安全性和对小鼠脾淋巴细胞增殖活性的影响,探讨菌株的免疫调节活性。试验结果表明:植物乳杆菌BF-15对人工消化液和胆盐有较强的耐受性,对Caco-2细胞黏附能力[(7.10±0.30)CFU/cell]显著高于阳性对照菌株LGG[(3.90±0.30)CFU/cell](P<0.05);BF-15除对氨基糖苷类、糖肽类抗生素的固有耐药性外,对苯唑西林、头孢噻吩也有耐药性,无抗性质粒;BF-15和LGG的活性和热致死菌在一定的菌浓范围(1×10~6~10~7CFU/mL)均促进体外小鼠淋巴细胞增殖,表现出剂量依赖关系,同时活性菌株作用效果明显优于热致死菌株。在相同菌浓条件下,两株菌体外免疫调节能力没有显著性差异(P>0.05)。母乳婴儿源乳杆菌——植物乳杆菌BF-15具有较强的抗逆性,较高的黏附性和一定的免疫调节能力。

    Abstract:

    The probiotic potential of four Lactobacillus from breast-fed infants faeces: Lactobacillus rhamnosus BF-1, Lactobacillus plantarum BF-15, Lactobacillus salivarius BF-29 and Lactobacillus paracasei BF-55 was evaluated by a series of in vitro assays: resistance to simulated gastrointestinal juice and bile, adhesion to Caco-2 cells, the safety and the effect on proliferative capacity of the spleen lymphocytes in th-e mice. Results showed that it was indicated that: Lactobacillus plantarum BF-15 has stronger tolerance toartificial digestive juice and bile salts. The adhesion to Caco-2 cells [(7.10 ± 0.30)CFU/cell] of BF-15 was significantly higher than the positive control strain LGG [(3.90 ± 0.30) CFU/cell](P<0.05). In addition, Lactobacillus plantarum BF-15 was resistant not only to aminoglycoside inherently,but also to oxacillin and cephalothin without plasmid. In a certain concentration range(1×106-107 CFU/mL), viable or heat-killed Lactobacillus both could promote lymphocyte proliferation of the mice in vitro and showed a dose-dependent relationship. Under the same bacterium concentration, there was no significant difference in immunoregulation ability between the two strains (P>0.05). Lactobacillus plantarum BF-15 presents favorable strain-specific properties, which could provide probiotic resources for developping new infants and young children formula milk powderin and new probiotic products in the future.

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张娜;张波;赵丽娜;王妙姝;康红艳;田洪涛;罗云波;.母乳婴儿源乳杆菌的筛选及其免疫调节能力研究[J].中国食品学报,2020,20(5):29-35

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