红参对乳酸菌体外增菌的影响
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河北省自然科学基金项目(C2017204094)


Effects of Red Ginseng on the Proliferation of Lactic Acid Bacteria in Vitro
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    摘要:

    采用比浊法和活菌计数法研究在基础培养基/菊芋汁基础培养基中添加不同量低聚木糖、低聚果糖、红参提取液及其酸水解物对鼠李糖乳杆菌AS 1.2466~T/保加利亚乳杆菌ATCC 11842生长情况的影响。结果表明:不同添加物对鼠李糖乳杆菌AS 1.2466~T的增菌效果为红参提取液>红参酸水解物>低聚木糖>低聚果糖。当红参提取液添加量为12.50%时,鼠李糖乳杆菌AS 1.2466~T的最大OD_(600)值为1.172,活菌数为2.72×10~8CFU/mL,比基础培养基中最大活菌数7.75×10~7CFU/mL增加1个数量级。对保加利亚乳杆菌ATCC 11842的增菌效果为红参提取液>低聚木糖>红参酸水解物>低聚果糖。当红参提取液添加量为6.25%时,保加利亚乳杆菌ATCC11842的最大OD_(600)值为0.627,活菌数为4.43×10~8CFU/mL,比菊芋汁基础培养基中最大活菌数1.48×10~7CFU/mL增加1个数量级。红参提取液比低聚木糖、低聚果糖具有较好的增菌效果,将红参作为益生元研究其益生功能,为研究红参对肠道微生物的影响提供试验依据。

    Abstract:

    In this study, the turbidimetry and viable count methods were used to study the growth of Lactobacillus rhamnosus AS 1.2466T / Lactobacillus bulgaricus ATCC 11842 in basic medium / Jerusalem artichoke juice base medium with different amounts of xylo-oligosaccharides, oligosaccharides, red ginseng liquid and its acid hydrolyzate. The results showed that the enrichment effect of different additives on Lactobacillus rhamnosus AS 1.2466T is red ginseng liquid medicine> red ginseng acid hydrolyzate> xylo-oligosaccharide> oligofructose. The OD600 value of Lactobacillus rhamnosus AS 1.2466T was 1.172 when the amount of red ginseng was 12.50%; and the number of viable cells was 2.72 × 108 CFU / mL, which was an order of magnitude higher than that of the basic medium 7.75 × 107 CFU / mL. The enrichment effect of prebiotics on Lactobacillus bulgaricus ATCC 11842 is red ginseng liquid medicine> xylo-oligosaccharides> red ginseng acid hydrolyzate> oligofructose. The OD600 value of Lactobacillus bulgaricus ATCC 11842 was 0.627 when the amount of red chemical solution was 6.25%; and the number of viable cells was 4.43 × 108 CFU / mL, which was an order of magnitude higher than the maximum viable count of 1.48 × 107 CFU / mL in the basal medium of Jerusalem artichoke. In summary, we found that red ginseng has a better enrichment effect than xylo-oligosaccharides or oligofructose. It can be used as a prebiotics to further study its prebiotics function, which can provide a basis on the study of the effect of red ginseng on intestinal microbes.

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邹美娟;孙永胜;李晨;康红艳;王妙姝;田洪涛;.红参对乳酸菌体外增菌的影响[J].中国食品学报,2020,20(5):36-42

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  • 在线发布日期: 2020-05-28
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