Tyr-Phe-Cys-Leu-Thr（YFCLT）， a corn peptide identified from corn gluten meal， was used as the research object. Through the in vitro anti-oxidation test and cell model test， the protective effects on HepG2 cells were studied from two aspects of oxidative stress and lipid metabolism. The results of antioxidant test in vitro showed that Tyr-Phe-Cys-Leu-Thr had DPPH radical scavenging ability， ABTS radical scavenging rate， iron chelating ability and oxygen radical absorbing ability （ORAC） at a lower concentration of 1 μmol/L. Alcohol was used as the inducer to induce the alcoholic injury model of human hepatocarcinoma cells （HepG2）. The optimal concentration of alcohol was 500 mmol/L for 24 hours. The results showed that Tyr-Phe-Cys-Leu-Thr could protect the HepG2 cells from oxidative stress injury by reducing the leakage of ALT and AST， the activity of SOD， cat and GSH， and the release of ROS and TNF-α. Tyr-Phe-Cys-Leu-Thr can also reduce the content of TG in cells and the accumulation of fat， thus play a protective role in lipid metabolism. In conclusion， Tyr-Phe-Cys-Leu-Thr can protect HepG2 cells from alcohol induced injury.