Metabolic characteristics and metabolic flow of Zygosacharomyces mellis LGL-1 under high- glucose stress were studied. Metabolites accumulated under 100 g/L and 400 g/L glucose concentration， such as alcohol， trehalose， glycerin， malic acid， α-ketoglutarate， citric acid， and lactic acid were determined. Metabolic flow distribution of key nods were analyzed through construction of metabolic matrix equations， MATLAB software and normalization method. The results showed that the carbon flow of Z. mellis LGL-1 was towards the metabolism branch of trehalose， glycerol， alcohol synthesis and TCA cycle. Compared with the normal cultivation， the metabolic flux of trehalose， glycerol and TCA under high-glucose stress was increased by 50.00%， 17.73% and 4.79%， respectively. But the metabolic flux of ethanol showed little difference. Trehalose and glycerin accumulation， flux change of TCA cycle under high-glucose stress reflected the important protective function to Z. mellis LGL-1.