On the basis of single factor experiments，the paper deals with the optimization of the synthesis conditions for the fructose glucose and glycine reaction synthesis of 1- deoxy -1-L- glycine -D- （Fructosyl-Glycine， Fru-Gly） using response surface methodology. Fru-Gly compounds were synthesized under different reaction time， reaction temperature and molar ratio of glucose to amino acid. The antioxidant capacity was evaluated by 1，1-diphenyl-2-picrylhydrazyl （DPPH） and 2，2’-azino-bis （3-ethylbenzothiazoline-6-sulfonic acid） diammonium salt （ABTS） radical scavenging capacity， and reducing power. The results showed that， the concentration of Fru-Gly compound in the condition which was reacting for 0.9 h at the reaction temperature 79 ℃ after the molar ratio of glucose to glycine controlled to 3.25 ∶ 1 was the highest. And at this time the antioxidant activity of the sample was the strongest. The correlation analysis showed that the concentration of Fru-Gly compounds was significantly correlated with DPPH radical scavenging force， ABTS radical scavenging force and reduction force， and the correlation coefficient was greater than 0.96.