N-乙酰半胱氨酸对槲皮素稳定性的影响及两者联合抗肿瘤活性评价
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国家重点研发计划(2017YFD0401202);国家自然科学基金面上项目(31571766);江苏高校优势学科建设工程资助项目(PAPD)


The Effect of N-acetylcysteine on the Stability of Quercetin and the Evaluation of Antitumor Activities for Quercetin Combined with N-acetylcysteine
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    摘要:

    为探究N-乙酰半胱氨酸(NAC)能否提高槲皮素在细胞培养液中的稳定性及两者联用能否产生更强的抗肿瘤活性,本文用高效液相色谱定量槲皮素在培养液中的实时浓度,MTT法评价槲皮素联合NAC的体外抗肿瘤效果。试验结果表明,槲皮素在细胞培养液中的降解符合一级降解动力学特征(R2>0.95),降解常数kobs与槲皮素的初始浓度无关,与NAC的浓度呈负相关,NAC能提高槲皮素在培养液中的稳定性。此外,槲皮素联合NAC对HepG-2、Caco-2、MKN-28和MDA-MB-231细胞的抗肿瘤活性均明显强于其各自作用时的抗肿瘤活性,其中对HepG-2细胞的联合抗肿瘤活性又明显强于对其它3种细胞的联合抗肿瘤活性。

    Abstract:

    In order to explore whether N-acetylcysteine (NAC) can improve the stability of quercetin in cell culture medium and whether quercetin combined with NAC can show stronger antitumor activity, the real-time concentration of quercetin in culture medium was determined by high performance liquid chromatography, and the degradation equation was fitted. Then the antitumor activity of quercetin combined with NAC in vitro was evaluated by MTT method. The results showed that the degradation process of quercetin in cell culture medium accorded with the first-order kinetic characteristics (R2>0.95). The degradation constant kobs was independent of the quercetin’s initial concentration and negatively correlated with the NAC concentration. So NAC could improve the stability of quercetin in culture medium. In addition, quercetin combined with NAC showed stronger antitumor activity of HepG-2 cells, MKN-28 cells, MDA-MB-231 cells and Caco-2 cells than the single use of quercetin or NAC. Among these four cells, NAC combined with quercetin showed strongest antitumor activity of HepG-2 cells.

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王立峰;陈 琳;姚轶俊;李枝芳;王海鸥. N-乙酰半胱氨酸对槲皮素稳定性的影响及两者联合抗肿瘤活性评价[J].中国食品学报,2020,20(6):19-26

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  • 在线发布日期: 2020-07-07
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