超高压处理条件对腐败希瓦氏菌的影响
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农业部海水鱼产业体系(CARS-47);2016年上海市科技兴农重点攻关项目[沪农科攻字(2016)第1-1号];上海市科委平台能力建设项目(16DZ2280300);上海市科委公共服务平台建设项目(17DZ2293400)


Effect of High Hydrostatic Pressure (HHP) Treatment Conditions on Shewanella putrefaciens
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    摘要:

    为研究不同压力条件的超高压处理对水产品特定腐败菌——腐败希瓦氏菌的影响以及对细胞损伤、致死的机制,分别对腐败希瓦氏菌菌体进行压力条件为200,300与400 MPa,保压时间9 min的超高压处理,并以常压(0.1 MPa)处理为对照组。分别测定细菌存活数、细菌生长曲线、电导率、碘化丙啶摄入量、核酸和蛋白质吸收量与酶活力等指标,并通过扫描电镜观察,综合评价不同压力条件对腐败希瓦氏菌菌体的影响。结果表明:与未经处理的菌体相比,腐败希瓦氏菌在不同压力的超高压处理后,其正常生长受到明显抑制,菌体对数生长期相应延迟。300 MPa与400 MPa高压处理能完全杀灭细菌。电导率值与碘化丙啶摄入量与压力呈正相关,而细胞膜中Na+/K+-ATPase活性明显下降。细菌超微结构观察表明,随着压力的增大,腐败希瓦氏菌菌体扭曲变形,菌体表面粗糙,且有细胞内容物外渗,菌体细胞均出现不同程度的损伤,最终导致细菌死亡。结论:超高压处理能够抑制腐败希瓦氏菌菌体生长,使其细胞膜受损,菌体活性受阻,最终导致其死亡。

    Abstract:

    The aim of this study was to investigate the mechanism of high hydrostatic pressure (HHP) on the dominant spoilage bacteria in aquatic products-Shewanella putrefaciens and to understand the changes in bacterial cell damage and death. The treatment conditions were set at 200, 300 MPa and 400 MPa for 9 min respectively; samples treated by 0.1 MPa were used as controlled one. Different parameters, such as survival rate, bacterial growth curve, electrical conductivity, propidium iodide(PI) intake, the content of nucleic acid and protein, enzymatic activity, were analyzed respectively. Scanning electron microscopy (SEM) can be used to observe the damage of Shewanella putrefaciens under different HHP conditions. The results showed that when compared with CK group, the growth of Shewanella putrefaciens could be greatly inhibited after different HHP treatment and the logarithmic phase of bacteria can be delayed. 300 MPa and 400 MPa for 9 min treatments could kill all of the bacteria. Electrical conductivity and PI intake were positive correlation with the pressure, but the activity of Na+/K+-ATPase in bacterial cell membrane was significantly decreased. The results of SEM observation showed that the level of deformation and cell damage were related to the increase of pressure. HHP treatment can inhibit the growth of bacteria, destroy its structure, inactive the enzymatic activity, which led to the death of bacteria.

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蓝蔚青;张 溪;赵宏强;孙晓红;施玲慧;丛晓涵;谢 晶.超高压处理条件对腐败希瓦氏菌的影响[J].中国食品学报,2020,20(6):122-128

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  • 在线发布日期: 2020-07-07
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