大孔树脂-聚酰胺分离纯化红树莓籽低聚原花青素及其体外模拟胃、肠消化
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国家重点研发计划专项(2017YFD0400704);辽宁省高等学校产业技术研究院重大项目(000041803)


Separation and Purification of Oligomeric Proanthocyanidins from Red Raspberry Seeds by Macroporous Adsorption Resin and Polyamide and Its Simulated Gastrointestinal Digestion in Vitro
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    摘要:

    采用大孔树脂对红树莓籽原花青素进行分级及初步纯化,再利用聚酰胺对低聚原花青素进行二次纯化。结果表明,吸附红树莓籽原花青素粗提物的大孔树脂经40%乙醇洗脱得到低聚原花青素,再经60%乙醇洗脱得到高聚原花青素。聚酰胺二次纯化最适参数为:上样液质量浓度2 mg/mL,上样流速1.5 mL/min,上样液体积100 mL;解吸剂乙醇体积分数70%,解吸流速1.5 mL/min,解吸剂体积150 mL,低聚原花青素纯度从52.36%提高到71.09%。模拟胃消化0~2.5 h和肠消化0~3.0 h,低聚和高聚原花青素释放量逐渐升高至14.23,5.02 mg/g和27.79,12.62 mg/g,表明胃蛋白酶、胰酶和胆汁可促进低聚及高聚原花青素的释放。胃、肠消化过程中,低聚原花青素释放量均远超高聚原花青素,说明低聚原花青素更利于释放,更利于人体吸收利用。

    Abstract:

    Macroporous adsorption resins were used to purify the crude proanthocynidins extract from red raspberry seeds, and then the polyamide was used for secondary purification of the oligomeric procyanidins. The results showed that the macroporous resin adsorbing the crude extract of proanthocyanidin from red raspberry seed was eluted with 40% ethanol to obtain oligomeric procyanidins, which were then eluted with 60% ethanol to obtain high polymeric proanthocyanidins. The optimum parameters for polyamide were determined as follows: sample concentration 2 mg/mL, flow rate 1.5 mL/min, sample volume 100 mL; 70% ethanol as the eluent, flow rate 1.5 mL/min, eluent volume 150 mL, the purity of oligomeric proanthocyanidins increased from 52.36% to 71.09%. Simulated gastric digestion 0-2.5 h and intestinal digestion 0-3.0 h, The release of oligomeric proanthocyanidins and high polymeric proanthocyanidins gradually increased to 14.23, 5.02 mg/g and 27.79, 12.62 mg/g, which indicated that pepsin, pancreatin and bile promoted the release of oligomeric proanthocyanidins and high polymeric proanthocyanidins from red raspberry seeds during simulated gastrointestinal digestion. The release of oligomeric procyanidins was much higher than that of high polymeric proanthocyanidins, indicating that oligomeric procyanidins were more beneficial to human body absorption and utilization.

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纪秀凤;芦 宇;刘海春;赵倩华;王维民;王贵虹;励建荣;吕长鑫.大孔树脂-聚酰胺分离纯化红树莓籽低聚原花青素及其体外模拟胃、肠消化[J].中国食品学报,2020,20(6):173-180

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  • 在线发布日期: 2020-07-07
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