With the growing threat to human and animal health aggravated by heavy metals in environmental pollution， research in Pb2+ tolerance and adsorbent of edible yeast has received more concerns. However， the research is facing the limitation from heavy and costly separation and screening work. In this paper， a high throughput rapid activation， high Pb2+ tolerance and adsorbent yeast screening method based on Deep-well Plate （DWP） was established， and the relationship between strains’ Pb2+ tolerance and adsorbent before and after domestication was revealed. The results are as followed： All strains were isolated from 19 samples which were collected from heavy metal pollution area in Inner Mongolia， the high throughput screening indicated that 26 strains yeast in 104 isolated possess high tolerance of Pb2+ over than 5 000 mg/L， 4 Wickerhamomyces anomalus， 3 Rhodotorula mucilaginosa and 2 Metschnikowia chrysoperlae among them are usually used in human and animals. In these 9 strains， similar tolerance and adsorbent can be found between strains of the same genus， and their tolerance was upgraded after domestication； for Pb2+ removal rate， however， 5 of them were declined， 3 of them were increased and the remaining strain was not changed； meanwhile， correspondence between tolerance and adsorbent was not found in strains， removal rate of 2 low-tolerance Metschnikowia chrysoperlae can reach up to 92.56%， removal rate of 3 high-tolerance Rhodotorula mucilaginosa only 23.14%， and Pb2+ tolerance concentration of 4 Wickerhamomyces anomalus was in the scope of 6 000-7 000 mg/mL， and their removal was 78.94%-91.67%. The high throughput screening method established in this paper can also applied in other microorganisms’ heavy metal tolerance and adsorbent test， showed outstanding research and application potential.