The antibiotic susceptibility of lactic acid bacteria isolated from tilapia was evaluated in this study. Circular disc diffusion method was used to determine antibiotic susceptibility of these isolates to 13 most widely used antibiotics， including Penicillin， Ampicillin， Erythromycin， Kanamycin， Streptomycin， Gentamicin， Tetracycline， Doxycycline， Vancomycin， Chloramphenicol， Ciprofloxacin， Norfloxacin， Ofloxacin. The results show that these strains primarily presented resistance to seven antimicrobial agents： PEN， KAN， GEN， VAN， CIP， NOR and OFL. The number of resistant strains was 19（45.2%）， 31（73.8%）， 24（57.1%）， 34（81.0%）， 30（71.4%）， 34（81.0%）and 20（47.6%）， respectively. There was variation in susceptibility between the species. Multidrug resistance was identified in most isolates.