A novel immunochromatic method for rapid detection of C. sakazakii was established in this paper. Antibody-modified magnetic beads （MNPs-IgG） were used as capture probes to separate and enrich C. sakazakii， which were then bound to biotin-labeled C. sakazakii antibody （Biotin-IgG） and avidin-peroxidase （Avidin-HRP）. The resulting complex can react with the substrate 3，3'， 5，5'-Tetramethylbenzidine （TMB）. After the termination of the sulfuric acid， a linear relationship between the glucose concentration and the logarithm of the C. sakazakii concentration is obtained in the concentration range from 103-107 CFU/mL， and the regression equation is y=0.2353x-0.2307. In this study， a new method based on colorimetric determination of C. sakazakii was successfully designed and optimized. The LOD of 8.68×102 CFU/mL is calculated according to the IUPAC definition （S/N=3） and a spiked sample detection rate of 96%. The detection time does not exceed 2.5 h， which provides theoretical support for practical application and commercialization.