The effects of different concentrations of PV separated from egg yolk （0， 50， 100， 200 and 500 μg/mL） on the proliferation， cell cycle and cell apoptosis of RAW264.7 cells for different time were studied. It was found that low concentration of PV could promote proliferation and inhibit the apoptosis of RAW264.7 cells. The effect of 100 μg/mL PV concentration which can promote the proliferation and inhibit apoptosis is best. CCK-8 method was used to characterize the proliferation of RAW264.7 cells. It was found that the proliferation of RAW264.7 cells showed two aspects. The cell proliferation was promoted at low concentration， the cell proliferation was inhibited at high concentration. The results showed that the cell viability was 64%-76% higher than that of the control group at 100 μg/mL. By analyzing the effect of PV on the cell cycle， found that the percentage of PV in the S phase was significantly higher than that in the control group （P<0.05）， 100 μg/mL PV has the best effect. The apoptotic effects of PV group at 50， 100 and 200 μg/mL were significantly lower than that of control group. When the concentration of PV was 500 μg/mL， the apoptotic rate was higher than that of the control group， which indicated that low concentration of PV could inhibit the apoptosis and high concentration of PV could induce apoptosis. In addition， different concentrations of PV can significantly reduce the number of osteoclasts and the activity of TRAP （P < 0.05）， thus inhibiting the RAW264.7 cell differentiation to form osteoclasts， and in a concentration-dependent manner， the best inhibitory concentration was 200 μg/mL. In conclusion， PV was not by inhibiting the growth of RAW264.7 cells， but by inhibiting the differentiation of RAW264.7 cells significantly reducing the activity of TRAP， thereby inhibiting the formation of osteoclasts.