Quorum sensing （QS） systems in gram-negative bacterial are primarily mediated by N-acyl homoserine lactone（AHLs） signaling molecules. Signal molecules gradually accumulate as the bacterial density increases. When the signal molecule concentration reaches a threshold， cognate receptors bind signal molecules and trigger signal transduction， and the phenotypic expression of phenotypes such as bacterial biofilm formation， bioluminescence， plasmid conjugative transfer， and virulence factors has been induced. At present， the research on quorum quenching of gram-negative bacteria is mainly based on the external intervention of one of the three components of synthetase（I）， AHLs signal and receptor （R） in the QS system， and then the gene expression that inhibits bacterial phenotype is realized. This article first briefly introduces the structure， synthesis method， QS system and regulation mechanism of AHLs， review focuses on the quorum quenching（QQ） mediated by AHLs and the sources， types， signaling molecular types and degradation mechanisms of AHLs lactone， acyltransferase and oxidoreductase quorum quenching enzymes. This paper provides reference for controlling the quorum-sensing mechanism of gram-negative bacteria mediated by AHLs， and also proposes a new idea for controlling diseases caused by gram-negative bacteria through quorum quenching.