Rapid evaporative ionization mass spectrometry （REIMS） is a recently developed ambient mass spectrometric technique without any sample preparation. In this study， a real-time lipidomics detection method was developed for fast profiling of Antarctic krill oil on the basis of REIMS. The Antarctic krill oil was extracted using 95% ethanol from freeze dried Antarctic krill. The compounds inside were evaporated by a heating probe at 500 ℃， and the aerosol generated was transferred into the interface of mass spectrometer by a Venturi pump with nitrogen at 2 bar. In the interface， a coil of Kanthal A1 wire （1.1 Ω， 3 V） was set for ion collision. A syringe pump was used to inject the matrix solvent at a flow rate of 0.1 mL/min throughout experiments. The propan-2-ol， together with the aerosol， entered the mass spectrometer via a stainless steel MS inlet capillary. The results showed that the lipid ion peaks were generally produced in the m/z range of 200-900. There are generally two clusters， including the cluster of fatty acid ions at m/z 200-500 and the cluster of phospholipid ions at m/z 600-900. After statistic analysis and lipid identification， a total of 20 fatty acid molecular species and 26 phospholipid molecular species were found. [EPA-H]- （m/z， 21.59%） and [DHA-H]- （m/z 327， 14.44%） were the most abundant fatty acid ions， while [PG 36：7-H]- （m/z 763， 11.67%） and [PC O-38：3-choline]-/[PE O-38：3-NH3]- （m/z 737， 11.46%） were the major phospholipid ions. After method validation， it indicated that the proposed REIMS method was sensitive and precise for high-throughput lipidomics profiling of Antarctic krill oil， which could provide important theoretical basis for mass spectrometry workers and technical support for related enterprise and functional departments.