In recent years， probiotics have gained wide popularity due to their association with health and wellbeing. Based on the bibliometric evaluation approach， the number of literatures， source journals and research hotspots were compared and analyzed for probiotics-related papers in the Chinese National Knowledge Information （CNKI） database and the Web of Science（WOS） database. The results showed that the research hotspots of probiotics are mainly related to three areas： probiotics and human health， probiotics and foods， probiotics and breeding industry. But the publication volume of probiotics in CNKI was much lower than that in the WOS. This paper systematically analyzed the similarities and differences in research hotspots in the field of probiotics in order to provide references for the better development and future of probiotics industry in China.