Wheat protein hydrolysis rich in glutamine were obtained by two（PWGH） or three-enzymes （AWGH） combined hydrolyzed methods. Then their abilities in resisting the oxidative stress of intestinal cells （Caco-2） were studied after the digestion in vitro. The results showed that both of PWGH and AWGH could alleviate the decrease of cell survival rate， and reduce the degree of cells damage. Moreover， the hydrolysis still maintained their activities after digestion， but the digestion of wheat protein showed no effect on the cell survival rate. PWGH and AWGH both improved the cellular antioxidant capacity， with an increase of intracellular GSH and decrease of lipid oxidation of damaged cells. The PWGH still had the function after digestion， but AWGH had no effect on the cellular antioxidant capacity and the level of GSH on damaged cells after digestion. It indicated that some active fragment in AWGH might be damaged after digestion， but not for the digestion of PWGH. Besides， it is worthy to note although the digestion of wheat protein could reduce the lipid oxidation of damaged cells， it had no effect on the cellular antioxidant capacity and GSH level.