In the study， 55 strains of lactic acid bacteria which were isolated from Tibetan air-dried meat and Bameng Zhongqi beef and mutton samples were screened by lipid plate method， a strain TR1-1-3 of lipase with high lipase activity was isolated and identified as Lactobacillus helveticus by 16S rDNA molecular biology. The fermentation enzyme production conditions of the strain were optimized by Box-Behnken response surface method on the basis of single factor experiment （inoculation amount， temperature， pH value， culture time， metal ions）. The results showed that the optimal solution could be obtained by Box-Behnken quadratic multivariate regression model equation， the optimum conditions were inoculation amount 2%， 36.2 ℃， pH 5.84， 48 h. Under these conditions， the enzyme activity was 10.92 U/mL， which was higher than before optimization. Moreover， the inoculum had the greatest influence on the enzyme production of the strain， followed by pH value and temperature. The interaction of the three factors was more significant， while the metal ions had less effect on it. Fe2+ and Mn2+ activated the enzyme production and Ca2+， Mg2+， Cu2+ have different degrees of inhibition.