Listeria monocytogenes is an important foodborne pathogen with high mortality rates. A total of 437 samples with 4 kinds of agri-food （fresh meat， egg， milk and vegetable） were collected from supermarkets， farmers markets and production base in Shanghai. L. monocytogens were isolated from these samples by GB 4789.30-2010 standard method， and the isolates serogroup was identified by multiple PCR. Results showed that total detection rate of L. monocytogenes was 14.9% （65/437）. The percentage distribution of the isolates was 27.2% （61/224）， 3.4% （2/58）， 2.0% （1/51） and 1.0% （1/104） for raw meat， egg， milk and vegetable， respectively. All of 79 isolates were serotyped into five serogroups： 1/2a or 3a （55.7%， n=44）， 1/2c or 3c （21.5%， n=17）， 1/2b or 3b or 7 （17.7%， n=14）， 4a or 4c （3.8%， n=3） and 4b or 4d or 4c （1.3%， n=1）. This research represents the different prevalence of L. monocytogens in raw-foods in Shanghai， and partial isolates belong to high-pathogenicity. Our results will provide the fundamental data for epidemiological monitoring of L. monocytogenes in Shanghai.