高F值寡肽M2和M3对小鼠肠道微生态的影响
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浙江舟山群岛新区“5313”领军人才项目;浙江省自然科学基金项目(Y19C200016)


Effects of High F Value Oligopeptides M2 and M3 on Intestinal Microecology of Mice
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    摘要:

    本文探究了从金枪鱼碎肉中制备并分离纯化的2个高F值寡肽M2、M3对小鼠肠道菌群结构的影响。对小鼠分别灌胃M2、M3溶液,收集小鼠肠道内容物,提取DNA进行16s rRNA测序。采用相似性分析、韦恩图、从主成分分析、主坐标分析、组间群落差异分析分析小鼠肠道菌群的物种组成及其丰度分布情况。结果显示:3组小鼠的肠道菌群结构存在差异,而两个寡肽组的菌群差异较小,与空白组的菌群组成存在较大差异。两个寡肽组的拟杆菌门和硬壁菌门的总量分别为90.17%和91.73%,均高于空白组的88.20%。两个寡肽组科水平上的优势菌为毛螺菌科、理研菌科和普雷沃氏菌科,而空白组为紫单胞菌科。M2寡肽组的优势特有菌为无形小体科,M3寡肽组的优势特有菌为理研菌科、另枝菌属和甲基杆菌科,空白组的优势特有菌为紫单胞菌科和莫拉菌科,说明高F值寡肽M2、M3能够调节小鼠肠道菌群的结构,促使有益菌增殖, 从而改善小鼠肠道微生态。

    Abstract:

    In this paper, the effects of two high F oligopeptides, M2 and M3, which were prepared from tuna minced meat and purified, on the intestinal flora structure of mice were investigated. By intragastric solutions of M2 and M3, intestinal contents of mice were collected, and DNA was extracted for 16s rRNA sequencing. Species composition and abundance distribution of intestinal flora of mice were analyzed using Anosim, OTUvenn, OUT PCA, PCoA and LDA EffectSize. The results showed that the intestinal microflora structure of the mice in the three groups was different, but the microflora of the two oligopeptides groups were less different, and the composition of the microflora of the blank group was significantly different. The total amount of bacteroidetes and firmicutes in the two oligopeptides groups was 90.17% and 91.73%, respectively, both higher than 88.20% in the blank group. At the level of the two oligopeptides, the dominant bacteria were spirillaceae, riken and prevotella, while the blank group was plasmodiaceae. The dominant characteristic bacteria of the M2 oligopeptide group were the invisible corpuscular family, the dominant characteristic bacteria of the M3 oligopeptide group were the rikenellaceae, mycobacterium and the methylobacteriaceae, and the dominant characteristic bacteria of the blank group were the purple single cells and moraxaceae. The results indicated that high F value oligopeptides M2 and M3 could regulate the structure of intestinal flora of mice, promote the proliferation of beneficial bacteria, and thus improve the intestinal microecology of inflammatory mice, laying a foundation for the subsequent study on the anti-inflammation activity of the high F oligopeptin M3.

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王春利;王志高;王一凡;余新威;罗红宇.高F值寡肽M2和M3对小鼠肠道微生态的影响[J].中国食品学报,2020,20(11):18-25

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  • 在线发布日期: 2020-12-04
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