Objective： To clone and recombinantly express ornithine transcarbamoylase from Lactobacillus brevis and study its enzymatic properties. Methods： The gene cloning process was using Lactobacillus brevis as template which based on the gene sequence analysis. The recombinant protein was expressed in E. coli， and purified by Ni-NTA affinity chromatography column. By using arginine as substrate， the enzymatic properties were studied. Results： The optimal temperature of recombinant ornithine transcarbamoylase is 55 °C. It has excellent thermal stability at 4 °C and still retains more than 50% biological activity at 37 °C for 3 hours. However， after 40 minutes at 65 °C it will totally lose activity； the optimal pH value of the enzyme is 6.5， when the pH is alkaline， the enzyme activity decreases significantly； the enzyme possess highest activity under 5% mass fraction of NaCl and 5% volume fraction of ethanol， but the difference is not obvious， indicating recombinant ornithine transcarbamoylase has good salt tolerance and alcohol tolerance； the kinetic parameters Km is 1.58 mmol and Vmax is 5.88 mmol/min. Conclusion： The ornithine transcarbamoylase derived from Lactobacillus brevis has good temperature stability， salt tolerance and alcohol tolerance， and has certain application prospects in alcohol fermentation.