Antarctic krill is an important source of phospholipids， which is also a natural storage for phospholipid bioaccumulation. In this study， the phospholipids from Antarctic krill was extracted by enzyme-assisted Bligh & Dyer extraction. The influcing factors of the extraction rate， such as temperature， pH value， enzymatic hydrolysis time and the kind of enzyme were investigated. The structure composition of fatty acids was assayed by gas chromatography with the quantification and analytical identification of phospholipids by HILIC-QTOF/MS. An optimum phospholipid extractants of （3.66 ± 0.03）% was obtained by the typsin， the extraction temperature of 41 ℃， the pH value of 9 and the extraction time of 3 h. The main structure of fatty acid chains were C16:0 （31.58%）， EPA （31.94%）， DHA （20.64%）. Under the enzyme-assisted Bligh & Dyer extraction， the content of EPA and DHA chains were up to 52.58% with the higher percentage of unsaturated fatty acids. Three kinds of phospholipids， including Phosphatidyl cholines（PC）， Phosphatidyl ethanolamine （PE） and Phosphatidylinositol （PI）， were successfully separated by HILIC-QTOF/MS. After the ion source separation， 59 kinds of phospholipid molecules were totally detected， while the structure information of fatty acid chains were identified by database and secondary mass spectrometry. The results revealed that an abundance of EPA and DHA chains were existed in phospholipid molecules from Antarctic krill. In conclusion， this study provided the foundation for the development and utilization of Antarctic krill phospholipids.