In this paper， zein-arabic gum （AG） nanoparticles loaded with tocopherol （TOC） were prepared by antisolvent method. The effects of different zein ∶ AG ratios and the stirring speeds on the stability of zein-AG nanoparticles were investigated， and the effects of different pH and the different concentrations of salt were also investigated. The experimental results showed that the nanoparticles with zein ∶ GA ratio of 1 ∶ 1.5 and the stirring speed of 200 r/min were stable， and the particle size was 125.4 nm， the polydispersity index （PDI） was 0.19 and the zeta-potential was -32.8 mV. When the pH=3~9， and the concentration of salt ion was less than 20 mmol/L， the zein-AG nanoparticles were stable which proved that zein and AG were bound effectively and had a certain charge shielding effect. The stability of zein-AG nanoparticles loaded with TOC was significantly improved， and the removal capacity of DPPH， ABTS and superoxide anions were higher than that of zein-AG nanoparticles. Moreover， zein/AG-TOC nanoparticles could achieve slow-release in gastrointestinal simulation experiments.