OPO结构脂在模拟婴儿胃肠道消化环境中脂肪酸的释放特性
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哈尔滨市科技局科技创新人才研究专项资金(优秀学科带头人)(2016RAXXJ021)


Fatty Acid Release Characteristics of OPO Structural Lipids in a Simulated Digestive Environment of Infant Gastrointestinal Tract
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    摘要:

    为研究OPO(1,3-二油酸2-棕榈酸甘油三酯)结构脂在模拟婴儿胃肠道消化环境中脂肪酸释放特性,根据母乳脂肪酸组成特点,制备了OPO结构脂(O组)。以婴儿配方粉中常用植物油混合物(Z组)作对比,测定脂肪酸组成及Sn-2位脂肪酸分布情况,分析消化过程中脂肪酸释放率、粒径和zeta电位的变化,并测定了消化产物中游离脂肪酸和甘油单酯的组成。研究结果表明:相同处理条件下,O组粒径集中分布在波长198 nm处,Z组大多分布在波长229 nm处,粒径分布的不同反映在初始消化阶段脂肪酸释放率存在明显差异(P<0.05)。胃液模拟30 min后,O组脂肪酸释放率为12.83%,Z组为8.92%。经体外胃肠道消化后测得O组脂肪酸的最终释放率为62.67%,而Z组仅为51.83%。结论:O组和Z组Sn-2位脂肪酸的分布存在差异(P<0.05),薄层色谱法验证了O组Sn-2位棕榈酸的含量较高且与母乳更接近。在模拟体外消化时表现出更高的脂肪酸释放率。消化产物甲酯化后经气-质谱分析,O组消化后产物中游离不饱和脂肪酸和棕榈酸甘油单酯含量均高于Z组。将本试验获得的OPO油脂和混合植物油脂在体外的消化脂肪酸释放特性进行比较,可得出如下结论:OPO结构脂经消化后,其脂肪酸更有利于婴儿消化吸收利用。

    Abstract:

    The purpose of this paper was to investigate the fatty acid release characteristics of OPO structural lipids in simulating the gastrointestinal digestive environment of infants. According to the characteristics of fatty acid composition of breast milk, OPO structural lipids (O Group) were prepared in this study, and mixed vegetable oil (Z group) in infant formula was used for comparison. The fatty acid composition and the distribution of fatty acids at the Sn-2 position were measured. The changes in fatty acid release rate, particle size and zeta potential during digestion were analyzed, and the composition of free fatty acids and monoglycerides in the digested products was determined. The results showed that under the same treatment conditions, the particle size of the O group was concentrated at 198 nm, and the Z group was mostly distributed at 229 nm. The difference in particle size distribution reflected the significant difference in fatty acid release rate during the initial digestion stage (P<0.05). After 30 min of the simulated gastric fluid, the fatty acid release rate of the O group was 12.83%, and the Z group was 8.92%. After the in vitro gastrointestinal digestion, the final release rate of fatty acids in O group was 62.67%, while the final release rate of fatty acids in Z group was only 51.83%. The analysis concluded that there was a difference in the distribution of Sn-2 fatty acids in the O group and Z group (P<0.05). Thin-layer chromatography confirmed that the content of Sn-2 palmitic acid in the O group was higher and closer to that of breast milk, and showed higher fatty acid release rate when simulated in vitro digestion. After methylation of the digested product, GC-MS analysis showed that the content of free unsaturated fatty acid and palmitic acid monoglyceride in the O group was higher than that in the Z group. Therefore, comparing the digestion of OPO oils and mixed vegetable oils in vitro, it lays a theoretical foundation for the development of formulas that are more conducive to digestion and absorption of infants.

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王静;李春;曲国强;孙雪婷;刘丽波. OPO结构脂在模拟婴儿胃肠道消化环境中脂肪酸的释放特性[J].中国食品学报,2020,20(12):39-47

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  • 在线发布日期: 2021-01-12
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