Static digestion mode in vitro was used to digest egg ovotransferrin by the simulated gastric juice， small intestine fluid and small intestinal brush border membrane enzyme in infants and adults， respectively. Tricine-SDS-PAGE and MALDI-TOF-MS were used to analyze the digestion products at all stages to define the digestibility of ovotransferrin. It was shown that ovotransferrin could be digested by the simulated gastric juice， small intestine fluid and small intestinal brush border membrane enzyme in infants and adults. Regarding to the digestion in simulated gastrointestinal fluids in infants， the molecular weight of gastric digestion products were 5-15 ku and 30-50 ku. The small intestinal digestion products were 0.5-10 ku. At last， 0.5-0.7 ku peptides were produced by small intestinal brush border membrane enzyme. The molecular weight of the digested ovotransferrin by the simulated gastric fluid in adult was the same as that of infants， while 0.5-0.8 ku peptides were mainly produced in intestinal digestion. However， only 0.5-0.7 ku peptides were generated after further digestion by small intestinal brush border membrane enzyme. This work preliminarily defined the characteristics of in vitro gastrointestinal digestion of ovotransferrin， providing some experimental support for better understanding that ovotransferrin is more likely to cause egg allergy in infants.