In order to explore the absorption mechanism of soybean peptides with the biological activity， 22-NBD cholesterol micelle incubated Caco-2 cells were used as a high cholesterol model. Effects of three kinds of soybean peptides VAWWMY（VY - 6）， LPYPR （LR - 5）， and WGAPSL （WL - 6） on the cholesterol dissolution inhibition rate， absorb cholesterol capacity， and cholesterol transport were investigated. Results indicated that WL-6 had the highest cholesterol dissolution inhibition rate at 0.5 mmol/L concentration. However， the inhibition rate gradually decreased with the increasing concentrations. WL-6 could inhibit 14.2% of cholesterol uptaking by cells at the concentration of 2 mmol/L. For the cholesterol absorbing capacity， VY-6 and WL-6 had the best inhibition rate of 43.1% and 45.95% at the concentration of 2 mmol/L， respectively. LR-5 had the best inhibition absorbing rate of 45.7% at the concentration of 2.5 mmol/L， which indicated that WL-6 had the best cholesterol absorbing capacity and higher than simvastatin. In the experiment of cholesterol transport， WL-6 had the best inhibition rate of 30.7% at the concentration of 2 mmol/L. Above all， WL-6 had the best power at cholesterol lowering effect.