变性淀粉对白鲢鱼鱼糜凝胶特性和蛋白构象的影响
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国家自然科学基金项目(31701629,31972107); 辽宁省“兴辽英才计划”青年拔尖人才项目(XLYC1907040); 中国博士后科学基金项目(2016M592021)


Effect of Modified Starch on Gel Properties and Protein Conformation of Surimi from Silver Carp
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    摘要:

    为了研究原木薯淀粉、羟丙基淀粉、乙酰化二淀粉磷酸酯和醋酸酯淀粉对白鲢鱼鱼糜凝胶特性和蛋白构象的影响,分析不同淀粉添加量条件下,白鲢鱼鱼糜白度、凝胶强度、持水性、质构特性、储能模量(G')、化学作用力、蛋白二级结构和微观结构的变化规律。结果表明,添加淀粉可以显著提高鱼糜凝胶的持水性。当其添加量为1%时,可提高鱼糜凝胶的白度、凝胶强度、硬度和咀嚼度,且羟丙基淀粉的改善效果最佳。流变学特性表明淀粉在鱼糜制品中起到非活性填料的作用。添加少量淀粉可阻碍氢键的形成,促进疏水相互作用及离子键的形成,诱导鱼糜蛋白的构象由α-螺旋结构向β-折叠结构转变。4种淀粉添加量为1%时均可以得到更为致密的鱼糜凝胶网络结构,其中,添加羟丙基淀粉可以使鱼糜凝胶网络中孔洞的大小、分布更均匀。添加1%的羟丙基淀粉可用于改善白鲢鱼鱼糜的凝胶品质。

    Abstract:

    The changes of whiteness, gel strength, water holding capacity, texture characteristics, storage modulus (G'), chemical force, protein secondary structure and microstructure of surimi from silver carp were analyzed under different starch concentration, in order to study the effects of native cassava starch, hydroxypropylated starch, acetylated distarch phosphate and acetate starch on the gel properties and protein conformation of surimi. The results showed that starch could significantly improve the water holding capacity of surimi gel. 1% of additive content could improve the whiteness, gel strength, hardness and chewiness of surimi gel, and the improvement effect was the best for hydroxypropylated starch. Rheological properties showed that starch played an inactive filler role in surimi products. A small amount of starch could hinder the formation of hydrogen bond, promote hydrophobic interaction and ion bond formation, and induce conformation transition of surimi protein from a-helix to β-fold structure. A more compact surimi gel network structure could be obtained when four kinds of starch were added to 1%. However, hydroxypropylated starch made the size and distribution of pores in the surimi gel network more uniform. Therefore, 1% hydroxypropylated starch can be used to improve the gel quality of silver carp surimi.

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米红波;王聪;苏情;仪淑敏;李学鹏;励建荣;黄建联;丁浩宸;王琪;.变性淀粉对白鲢鱼鱼糜凝胶特性和蛋白构象的影响[J].中国食品学报,2021,21(1):72-80

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  • 在线发布日期: 2021-02-22
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