In this experiment， soybean was used as raw material， and the effects of buffer solution， aqueous phase and enzymatic methods on the extraction rate， composition， fatty acid composition， phospholipids， tocopherols， ζ-potential and particle size of soybean oil body were compared， and the effects on the oxidation stability of soybean oil body were analyzed. The results showed that the extraction rate of soybean oil body by enzymatic method（19.74 ± 0.14）% was significantly higher than that by buffer solution method （12.76 ± 0.14）% and aqueous method （6.67 ± 0.32）% （P<0.05）. There were significant differences in the composition of soybean oil body， fatty acid composition， phospholipid and tocopherol content， ζ-potential and particle size of soybean oil body extracted by different methods （P<0.05）. On the first day after storage at 60 ℃， the peroxide values of soybean oil body extracted by buffer solution， enzymatic method and aqueous phase method respectively were（1.67 ± 0.14），（1.84 ± 0.10），（1.64 ± 0.00） μg/mL， there was no significant difference（P＞0.05）. The TBARS of oil body extracted by aqueous phase method（1.14 ± 0.01） μg/mL was significantly higher than that by enzymatic method （1.02 ± 0.02） μg/mL and buffer solution method （1.09 ± 0.02） μg/mL （P<0.05）； the TBARS of soybean oil body extracted by buffer solution method was significantly higher than that by enzymatic method （P<0.05）； the acid value of oil body extracted by enzymatic method （0.71 ± 0.02） μg/mL was significantly lower than that by aqueous phase method （0.96 ± 0.13） μg/mL and buffer solution method （0.93 ± 0.09） μg/mL （P<0.05）. There was no significant difference between aqueous phase method and buffer solution method（P＞0.05）. Therefore， the composition and physicochemical properties of soybean oil body obtained by different extraction methods were significantly different（P<0.05）， the soybean oil body extracted by enzymatic method has the highest extraction rate， the highest fat content and the best stability.