In this study， we described the selection of DNA aptamers for β-phenethylamine using Capture-SELEX based on fixed ssDNA library， which is the technology of systematic evolution of ligands by exponential enrichment （SELEX）. During the Capture-SELEX progress， melting curve analysis was developed to monitor the enrichment of the aptamer species. The affinity and specificity of the aptamer were verified by graphene oxide（GO） fluorescence method. We obtained an aptamer （PHE-2） of β-phenethylamine with a Kd value of （71.64±11.47） nmol/L. The aptamer provides a new molecular recognition element for the analytical detection of β-phenethylamine.