To study the effect of different turgor treatment on micromechanics of parenchyma cells of Winter jujube. In this paper， mannitol solutions of 0.1， 0.45， 0.8 mol/L were used to treat the parenchyma of Winter jujube. The parenchyma cells of Winter jujube were in different turgor states. AFM was used to conduct nano indentation test on parenchyma cells of Winter jujube at different turgor. The effects of different turgor pressure on the young’s modulus， apparent stiffness and adhesion of tissue cells in Winter jujube were analyzed. The results showed that： The young’s modulus E under high turgor pressure and normal turgor pressure and low turgor pressure were respectively： （6.76±7.57） kPa， （20.21±16.95） kPa， （5.58±4.24） kPa. The young’s modulus E value of normal turgor had a significant difference from that of low and high turgor （P<0.05）. The apparent stiffness value k ranges from high turgor to low turgor： （0.013±0.003） N/m， （0.018±0.004） N/m， （0.008±0.002） N/m， and its variation trend was consistent with young’s modulus， indicating that young’s modulus E can reflect the apparent stiffness. Adhesion forces at high turgor， normal turgor and low turgor were （102.06±163.55） pN， （178.41±141.59） pN， （187.96±76.17） pN， respectively. The adhesion at high turgor pressure was significantly different from that at normal and low turgor pressure（P<0.05）. Different turgor pressure may lead to different micromechanical properties of parenchyma cells of Winter jujube.