To study the inhibitory effect of two antioxidants （phytic acid， L-ascorbyl palmitate） on α-glucosidase， and explore the interaction of the two compounds with the enzyme by molecular docking technique. The IC50 values of phytic acid and L-ascorbyl palmitate for α-glucosidase were （0.207±3.137） mol/L and （0.39±0.00838） mmol/L， respectively. The inhibitory effect of L-ascorbyl palmitate was better. The inhibition types of phytic acid and L-ascorbyl palmitate on α-glucosidase were reversible mixed inhibition and reversible competitive inhibition， respectively. Molecular docking results showed that the two compounds interact well with the amino acid of the α-glucosidase active site. L-ascorbyl palmitate exhibits high efficiency in the inhibition of α-glucosidase and provides a theoretical basis as an enzyme inhibitor. It can reduce the postprandial level of diabetic patients while playing an antioxidant role in food.