In order to determine the optimal extraction process of Ganoderma lucidum spore polysaccharide （GLSP）， the ratio of material to liquid， extraction time and extraction temperature were used as the indicators. The yield of polysaccharide extraction was based on the single factor test. The optimum method for extracting polysaccharides from Ganoderma lucidum spores was as follows： extraction temperature 81 ℃， ratio of material to liquid 19：1， extraction time 2 h， under which the extraction yield of Ganoderma lucidum spore polysaccharide was （1.507±0.028）%. Using Ganoderma lucidum spore （GLS） from 7 different sources as raw materials， the Ganoderma lucidum spore polysaccharide was extracted by the best process， derivatized by trifluoroacetic acid hydrolysis and saccharide acetate method， and then gas chromatography-mass spectrometry was used. The monosaccharide composition of Ganoderma lucidum spore polysaccharide was determined by GC-MS. The results showed that the polysaccharides of the seven Ganoderma lucidum spore polysaccharides were similar， consisting mainly of 6 monosaccharides， namely rhamnose， arabinose and xylose， mannose， glucose， galactose， but the content of monosaccharides in different regions was significantly different （P<0.05）， and the average molar ratio was 3.860， 9.829， 11.044， 22.078， 40.338， 14.273. In vitro antioxidant experiments showed that Ganoderma lucidum spore polysaccharide has certain antioxidant capacity， but there were significant differences between different sources. The overall antioxidant performance is S2>S3>S5>S4>S6>S7>S1.