In this study， yeasts were purified from traditional shrimp paste by gradient dilution method and their carbon source utilization ability were studied. After extraction of genomic DNA and PCR amplification， 26S rDNA sequence analysis for these yeasts were carried out. Four kinds of yeasts were identified， which were Rhodotorula mucilaginosa， Candida parapsilosis， Trichosporon domesticum and Debaryomyces hansenii， respectively. Carbon source utilization was evaluated with carbon source assimilation analysis. Results showed that the utilization ability of carbon source from strong to weak was as follow： glucose = sucrose> glucosamine> sorbitol> glycerol> mannitol> citric acid> lactose> chitosan. Glucose and sucrose were the optimal carbon sources， which can support the growth of all yeasts. Glucose and sucrose can support all yeast growth， and the remaining carbon sources can support yeast growth to varying degrees. This study provides the microbial resource from shrimp paste.