To explore the effects of functional sugars on intestinal hormone secretion， matrigel were used to induce endocrine differentiation of NCI-H716 cells to establish an endocrine model. This model was used to evaluate the ability of trehalose， erythritol， sorbitol， xylitol， isomalt and L-arabinose to promote the secretion of glucagon-like peptide-1 （GLP-1）， glucagon-like peptide-2 （GLP-2）， and peptide YY（PYY）. The results showed that trehalose and sorbitol could significantly promote the secretion of GLP-1 and PYY， erythritol could significantly promote the secretion of GLP-1 and GLP-2， xylitol could significantly promote the secretion of PYY， isomaltitol and L-arabinose had no significant effect on the secretion of the three hormones. Trehalose， erythritol and sorbitol were selected to further study the mechanism of GLP-1 secretion. It was found that they could stimulate GLP-1 secretion by activating genes such as T1R2、T1R3、Gα-gustducin、PLCβ2、TRPM5 and increasing intracellular Ca2+ concentration.