When the national standard GB 4789.10-2016 was used to isolate and identify Staphylococcus aureus from frozen meat and meat products， it was found that the interference of background flora was so serious to lead to a large number of false negative results. In order to solve this problem， the national standard method was modified as follows， 10% NaCl broth was used for pre-enrichment；then the suspicious S. aureus colonies on Baird-Parker agar plates were picked on tryptic soy agar plate containing 4% agar and 2% ethanol to be further purified， and the subsequent verification was carried out as the standard. Finally， the isolation rate of S. aureus was improved from 2.61% to 19.98%. This modified methord effectively reduce the occurrence of false negative results.