Because the traditional methods of protein purification need to go through separation， collection， purification and structure analysis， it is too cumbersome and time-consuming. In this paper， virtual enzymolysis and molecular docking technology were used to screen xanthine oxidase（XOD） inhibitory peptide in bass myosin， and ADMET properties were predicted by Discovery studio 2017 R2 client （DS2017） software， and molecular docking was carried out. Then， the activity of the selected peptide was verified by high performance liquid chromatography（HPLC） and superoxide radical inhibition kit. The HPLC conditions were： column temperature 45℃， mobile phase = V methanol ∶ V mixture（ V water ∶ V glacial acetic acid ∶ V tetrabutylammonium hydroxide = 997 ∶ 1.5 ∶ 1.5） = 5 ∶ 95， detection wavelength 254 nm， flow rate 0.5 mL/min， and injection volume 10 μL. Finally， a six peptide DEEIDN was successfully screened， its ADMET property was good， and it could be successfully combined with XOD. The activity verification results showed that it had good XOD inhibition rate， its semi inhibition concentration IC50 value was 0.503 mg/mL， and it could produce the activity unit of inhibiting superoxide anion free radical， so as to effectively remove the superoxide anion which caused damage to human body.