The world wine culture prevails， and the problem of the alcohol injury to people is more and more serious. The intervention effect of diffierent probiotic strains on alcohol injury need to be evaluated. During the experiment period， ICR mice were given 50% ethanol by intragastric gavage， and were divided into groups with Lactobacillus plantalum Zhang-LL （1.0×107 CFU/d in low-dose group， 1.0×109 CFU/d in high-dose group） and its culture supernatant to intervention. After 12 weeks of intervention， the alleviating effect of Lactobacillus plantalum Zhang-LL on the negative effects of long-term alcohol intake and body weight was evaluated. The results showed as follows： Lactobacillus plantalum Zhang-LL intervention could significantly alleviate the reducing of food intake and inhibited weight addtion（P<0.05）；Compared with the model group， the supernatant of Lactobacillus plantarii Zhang-LL significantly reduced the levels of triglyceride and low density lipoprotein cholesterol in serum， increased the content of glutathione in liver， decreased intestinal permeability， maintained the integrity of small intestinal villi， and balanced the structure of intestinal microflora in mice with alcohol injury（P<0.05）. In the test cycle， the pathological changes of liver tissue structure were not observed in alcohol injury mice. There were no significant changes in glutamate aminotransferase activity， aspartate aminotransferase activity， malondialdehyde content and glutathione peroxidase activity in tissue and blood， and there were no significant changes in these indexes in Lactobacillus plantoflora intervention groups（P>0.05）. Conclusions： Lactobacillus plantarium Zhang-LL can significantly improve some of the negative effects caused by alcohol intake， and its culture supernatant has the most significant effect.