Objective： To determine the evaluation model of cellular antioxidant activity for boletus polyphenols. Methods： Based on three different cells， human colon cancer cell Caco-2， human liver cell L02 and human liver cancer cell HepG2， the antioxidative activity of the boletus polyphenols was detected， and a suitable cellular antioxidant evaluation model was determined. Using a suitable cell model， cellular antioxidant activity of polyphenols from Boletus rubeus， Boletus auripes， Boletus nigricans was determined， and analysis correlation with the polyphenol extraction rate and oxygen radical absorbance capacity of boletus to verify the effectiveness and accuracy. Results： Three cell models could detect the cellular antioxidant activity from Boletus auripes polyphenols， but there were differences in the range and sensitivity of the detection. The detection limits of Caco-2， L02 and HepG2 models were 0.025， 0.1， 0.2 g polyphenols which were extracted from Boletus auripes respectively. Among them， detection limit of Caco-2 was the lowest and significantly better than L02 and HepG2 models. The Caco-2 cell model was used to determine the antioxidant capacity of three boletus polyphenols. The polyphenol extraction rate and ORAC correlation coefficients were 0.9662 and 0.9156 respectively. By significant correlation， the Caco-2 cell model was effective and accurate. Conclusion： The Caco-2 cell model was more suitable for detecting the cellular antioxidant activity of boletus polyphenols. It can be used to evaluate the antioxidant activity of boletus polyphenols by combine with ORAC and other chemical evaluation methods in vitro.