In order to investigate the diversity of endophytic bacteria in Kuqa almond and collect potential microbial resources. In this paper， Biolog-ECO microplate method and culture method was used to isolate and screen endophytic bacteria from Kuqa almond， and characteristics of enzyme production and antagonism of the strains were analyzed. The results showed that main utilization of carbon sources were carbohydrate and amino acid， accounting for about 77% of the total. D-galacturonic acid， D-Mannitol， N-acetyl-D-glucosamine and L-asparagine were major compounds utilized by endophytic bacteria. Meanwhile， 47 isolates belonged to 13 genera of Proteobacteria， Firmicutes， Actinobacteria and Bacteroidetes were obtained， and one potential new species was observed. Additionally， a number of bacteria producing protease， lipase， amylase， pectinase and antagonism was found. In a conclusion， diversity of endophytic bacteria in Kuqa almond was rich， which provided a research basis for utilization of related functional strains.