In order to understand the health function of Qingzhuan Maocha， and investigate the influence on gut microbiota intestinal microorganisms in high-fat diet fed mice. In this study， the main physiological and biochemical indexes of high-fat diet fed mice treated by Qingzhuan Maocha were compared and analyzed， and the structure and composition of gut microbiota were analyzed by using high-throughput sequencing technology. The results showed that Qingzhuan Maocha could significantly reduce the increase of body weight， mesenteric adipose tissue weight， liver weight and the main serum lipids （total cholesterol， triglyceride， high density lipoprotein-cholesterol， low density lipoprotein-cholesterol， alanine aminotransferase， aspartate aminotransferase） levels in high-fat diet fed mice. Meanwhile， Qingzhuan Maocha effectively alleviated liver enlargement， reduced the increase in the volume and number of fat vacuoles in the liver. Qingzhuan Maocha can regulate the gut microbiota disorder induced by high-fat diet， and has the similar composition of healthy gut microbiota with the low-fat diet. A total of 46 key differentially OTU were found that the OTU changed significantly after the high-fat diet， but was adjusted back by Qingzhuan Maocha. Among them， 39 OTU showed significant positive or negative correlation with at least one of the main physiological and biochemical indexes. Qingzhuan Maocha may alleviate metabolic syndrome induced by high-fat diet by adjusting the ratio of Firmicutes/ Bacteroidetes and the relative abundance of Erysipelotrichaceae， Coriobacteriaceae， Lachnospiraceae and Ruminococcacea.