The intestinal microbiota， acting as an important ‘Organ’ in the human body， is related to a diverse variety of physiological functions including immunity， nutrition and metabolism. Culture-independent metagenomic approaches clarify the complex relationship between intestinal microbiota and human health， but it fails to obtain bacterial isolates， the internal mechanism of specific bacteria playing a role in the intestine cannot be identified， and is not conducive to the further discovery of intestinal ‘dark matter’. Consisting of multiple culture conditions combined with the rapid identification of bacteria， the culturomic approach has enabled the culture of hundreds of new microorganisms that are associated with humans， providing a pure culture of microorganisms for in-depth study of bacterial phenotypes and gene functions， as well as screening new probiotics of intestine provide data support. This review summarized the development of culturomics in recent years and its advantages to the study of human intestinal microbiota.