丹皮酚对格氏沙雷菌群体感应现象及生物被膜形成的影响
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国家自然科学基金项目(31972107,31471639); 国家重点研发计划项目(2019YFD0901702)


Effect of Paeonol on Quorum Sensing Phenomenon and Biofilm Formation of Serratia grimessi
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    摘要:

    采用生物传感器紫色杆菌CV026检测丹皮酚的群体感应现象抑制能力,探讨丹皮酚对格氏沙雷菌群体感应现象及生物被膜形成能力的影响。结果表明:在亚抑菌浓度下,丹皮酚对紫色杆菌CV026产紫色菌素有抑制作用,500μg/mL丹皮酚处理紫色杆菌后紫色菌素产量下降57.95%。光学显微镜分析丹皮酚对格氏沙雷菌生物被膜形成有抑制作用,扫描电镜验证丹皮酚不仅抑制生物被膜形成,而且可使生物被膜断裂。丹皮酚还抑制格氏沙雷菌毒力因子的表达,如蛋白酶和胞外多糖产量,随着丹皮酚质量浓度的增加,抑制作用呈现质量浓度依赖性。由此可见,丹皮酚有较强的群体感应抑制效果。

    Abstract:

    In this paper, bioreporter strain Chromobacterium Violaceum 026 (CV026) was used to detect the inhibitory effect of quorum sensing inhibition activity of paeonol. Then anti-quorum sensing and anti-biofilms efficacy of paeonol against Serratia grimessi were further studied. The results indicated that peaonol could inhibit the violacein production secreted from CV026 when the bacteria was the sub-minimum inhibitory concentration (sub-MIC). When peaonol mass concentration was 500 μg/mL, the yield of violacein decreased by 57.95%. The results of optical microscope analysis showed that paeonol could hinder the biofilm formation of Serratia grimessi. The scanning microscope results also manifested the biofilm inhibition of paeonol and further showed a rupture of biofilm caused by paeonol. Furthermore, the expression of virulence factors, such as swarming activity, protease production and extracellular polysaccharide production, had been suppressed after treating with paeonol. With the increase of paeonol, the suppressed effect was shown as the mass concentration-dependently. These results demonstrated that the paeonol had an effective inhibitory activity and could be developed as a potential quorum sensing inhibitor (QSI).

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刘景云;李婷婷;王当丰;杨亚茹;励建荣;谢晶;王明丽;周小敏;郭晓华;.丹皮酚对格氏沙雷菌群体感应现象及生物被膜形成的影响[J].中国食品学报,2021,(8):25-34

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  • 在线发布日期: 2021-09-19
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